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The Escherichia coli strain XL1-Blue was employed as a host for cloning the N-TPL PCR fragment in the P.pastoris transfer vector pGAPZaA (Invitrogen). The P.pastoris host pressure was X33 (wildtype pressure from Invitrogen)

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Deletion of the C-terminal area diminished each the sum of truncated mutant protein in...
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In some experiments cells had been addressed with 1 mM DMOG (Alexis, Grunberg, Germany). ?L929 HIF-1a knock down clones were being generated by lentiviral transduction with the pLKO

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HIF-1a knock down has an effect on cytoplasmic actin reorganisation in hypoxia. ShC cells,...