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These final results suggest that phosphorylated PPPSPXS peptide inhibited b-catenin phosphorylation by GSK3 through a manner that is impartial of PPPSPXS-binding to Axin, most likely by inhibiting GSK3 straight

In vitro reconstitution of Axin-dependent and CK1 priming-dependent b-catenin phosphorylation by GSK3. A. Recombinant...
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PCR reactions were executed in the Used Biosystems 7900HT Sequence Detection System (Applied BioSystems, Usa) in accordance to the manufacturer’s protocol. All experiments (n=three) were performed in triplicates

Locked nucleic acid-modified oligonucleotide (Exiqon, Vedbaek, Denmark) probes (miRBase version twelve.) had been utilised...