Reoriented subtstacks generated in VaaD are shown. For each LCa and LCd,comparable final results had been observed for LC cells from five optic lobes,every single with no less than three labeled cells. Analyses of MCFOlabeled LCb ( cells from brains) and LCc ( cells from brains) single cells also showed an approximate correspondence involving AP positions of dendrites inside the MedChemExpress (-)-DHMEQ lobula and DV positions of axonal arbors in the AOTu. LCb cells also showed considerable variation in their lateralmedial spread within the medial zone with the AOTu but further analyses is going to be necessary to explore feasible correlations among these differences and arbor positions within the lobula. Scale bars represent mm (A,D,E,H,K) or mm (C,F,G,I,L). DOI: .eLife The following figure supplement is available for figure : Figure supplement . Terminal arbor arrangements of further LC cell forms. DOI: .eLifean example of a cell form that appeared to retain some retinotopy,though with pretty low spatial resolution,at the amount of the axonal terminals: terminals of single LC cells expanded via only part of the glomerulus and their position correlated using the approximate position in the corresponding dendrites within the lobula (Figure D.Wu et al. eLife ;:e. DOI: .eLife. ofResearch articleNeuroscienceA A BB A BC A BLCLC A BDLCLCLCLCLCLCLCLC A BLCALCLCLCLCLCLPLCLPLC A BEFLCLCLCaLCbLCcLCdFigure . Layer certain arborizations of LC neurons inside the lobula. (A) AntiBrp neuropil marker shows PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25352391 bands of various intensity in the lobula that will serve as approximate markers of layer boundaries. The image can be a maximum intensity projection by way of adjacent sections mm spacing) of your reference channel in the normal brain used for alignments. Approximate layer boundaries are indicated. Layer boundaries were defined by the positions of recognized cell sorts and closely match the antiBrp pattern (see Figure figure supplement. Lo was divided into two sublayers depending on the antiBrp pattern. Further subdivisions of strata based on the positions of arbors of different cell types would be probable but have been not applied here. (B,C) Layer patterns of LC (B) and LC (C). SplitGAL expression of a membrane marker is shown in green. Pictures have been aligned to a typical brain utilizing the antiBrp pattern (shown in grey). Images are maximum intensity projections through the exact same series of sections of brains aligned towards the similar template as in (A). Approximate layer boundaries are marked with white lines. (D) Layer patterns on the remaining LC cell varieties (except LC neurons). Projections were generated as in (B,C) but are shown without the need of the antiBrp pattern. All layers,but only a portion in the lobula is shown. Schematics in (B indicate innervated layers as filled circles; black circles represent much more extensive arborizations than grey circles. Note that these simplified schematics do not capture some specifics of the layer patterns (such as sublayer patterns). An more description of layer patterns is usually discovered in Supplementary file A. (E) Single cell layer patterns are constant with layer patterns observed at the population level. LC and LC are shown as examples. Added single cell pictures is often found in Figure figure supplement . (F) Layer patterns of LC subtypes. LCb and LCd cells have comparable layer patterns but differ in other aspects such as arbor size (LCb arbors inside the lobula are larger). Extra examples of MCFO labeled LC cells of unique subtypes could be identified in Figure figure supplement and Figure figure supplement.
