Were observed (x2 = 1.864, P = 0.601), their gender ratios were significantly different (x2 = 9.352, P = 0.025). Among the genotype 1, 2, and 3 groups, the male percentages were similar (73.9 , 73.9 and 75.7 , respectively), but they were significant lower than the percentage in the genotype 6 group (90.0 ). Taken together, among the four genotype groups and excluding gender, the donors’ age and ethnicity were statistically matched.Correlation between viral loads, HCV genotypes, and the donors’ genderUnivariate analysis was performed to analyze the correlation between the viral loads and the detected HCV genotypes. The detail viral loads data can be found in Table S2. Since there was a higher proportion of male ��-Sitosterol ��-D-glucoside donors in the genotype 6 group while the male gender has been reported to be a risk factor for sustaining 
higher levels of the virus [29], a correlation between the viral loads and the donors’ gender was also analyzed. As shown in Table 3 and Figure 1, statistical analyses revealed that the viral loads were significantly different between the male and female donors and among the four genotype groups. The mean viral load among male donors was 6.06 log10 IU/ml comparing to 5.69 log10 IU/ml among female donors (t = 2.785, P = 0.006). Among the four genotype groups, significant differences in viral loads were also found (F = 6.675, P,0.001). Donors infected with genotype 1 and genotype 6 showed higher mean viral loads, which were 6.07 and 6.15 log10 IU/ml, respectively. However, among donors 
infected with genotype 2 and genotype 3 the viral loads were lower, which were 5.66 and 5.49 log10 IU/ml, respectively. These results indicated that male donors and donors infected with genotype 1 and 6 were more ML 281 custom synthesis likely to have higher loads of HCV. Higher viral loads have been reported among patients infected with genotype 1 than with genotype 2/3 [19,20,21]. In this study, we observed higher viral loads not only in the genotype 1 group but also in the genotype 6 group, when comparing with those in the genotype 2 and 3 groups. However, since a higher percentage of male donors was found in the genotype 6 group than in the genotype 1, 2, and 3 groups while the male gender showed higherviral loads than female gender, a question was asked if the higher viral loads we observed among the donors infected with genotype 6 was contributed mainly by HCV genotype or by donors’ gender. To answer this question, a multivariate regression analysis was performed under the generalized linear model. As shown in Table 4, both the donors’ age and ethnicity were not correlated with the viral loads (P = 0.973 and 0.212, respectively), while the donors’ gender and HCV genotype were (P = 0.031 and 0.011, respectively). We also compared the viral loads among male donors (Table 5). In agreement with the results previously described [19,20,21], the viral loads among male donors infected with genotype 1 or 6 were significant higher than those with genotype 2 or 3, with the mean values of 6.18, 6.18, 5.63, and 5.59 log10 IU/ml, respectively (F = 5.501, P = 0.001). In conclusion, both multivariate regression analysis and stratified analysis confirmed that independently the viral loads were correlated with the detected HCV genotypes and the donors’ gender.DiscussionIn this study, the viral loads of HCV were analyzed among a cohort of voluntary blood donors who were HCV viremic. It was revealed that both the detected HCV genotypes and the donors’ gender are two independent fac.Were observed (x2 = 1.864, P = 0.601), their gender ratios were significantly different (x2 = 9.352, P = 0.025). Among the genotype 1, 2, and 3 groups, the male percentages were similar (73.9 , 73.9 and 75.7 , respectively), but they were significant lower than the percentage in the genotype 6 group (90.0 ). Taken together, among the four genotype groups and excluding gender, the donors’ age and ethnicity were statistically matched.Correlation between viral loads, HCV genotypes, and the donors’ genderUnivariate analysis was performed to analyze the correlation between the viral loads and the detected HCV genotypes. The detail viral loads data can be found in Table S2. Since there was a higher proportion of male donors in the genotype 6 group while the male gender has been reported to be a risk factor for sustaining higher levels of the virus [29], a correlation between the viral loads and the donors’ gender was also analyzed. As shown in Table 3 and Figure 1, statistical analyses revealed that the viral loads were significantly different between the male and female donors and among the four genotype groups. The mean viral load among male donors was 6.06 log10 IU/ml comparing to 5.69 log10 IU/ml among female donors (t = 2.785, P = 0.006). Among the four genotype groups, significant differences in viral loads were also found (F = 6.675, P,0.001). Donors infected with genotype 1 and genotype 6 showed higher mean viral loads, which were 6.07 and 6.15 log10 IU/ml, respectively. However, among donors infected with genotype 2 and genotype 3 the viral loads were lower, which were 5.66 and 5.49 log10 IU/ml, respectively. These results indicated that male donors and donors infected with genotype 1 and 6 were more likely to have higher loads of HCV. Higher viral loads have been reported among patients infected with genotype 1 than with genotype 2/3 [19,20,21]. In this study, we observed higher viral loads not only in the genotype 1 group but also in the genotype 6 group, when comparing with those in the genotype 2 and 3 groups. However, since a higher percentage of male donors was found in the genotype 6 group than in the genotype 1, 2, and 3 groups while the male gender showed higherviral loads than female gender, a question was asked if the higher viral loads we observed among the donors infected with genotype 6 was contributed mainly by HCV genotype or by donors’ gender. To answer this question, a multivariate regression analysis was performed under the generalized linear model. As shown in Table 4, both the donors’ age and ethnicity were not correlated with the viral loads (P = 0.973 and 0.212, respectively), while the donors’ gender and HCV genotype were (P = 0.031 and 0.011, respectively). We also compared the viral loads among male donors (Table 5). In agreement with the results previously described [19,20,21], the viral loads among male donors infected with genotype 1 or 6 were significant higher than those with genotype 2 or 3, with the mean values of 6.18, 6.18, 5.63, and 5.59 log10 IU/ml, respectively (F = 5.501, P = 0.001). In conclusion, both multivariate regression analysis and stratified analysis confirmed that independently the viral loads were correlated with the detected HCV genotypes and the donors’ gender.DiscussionIn this study, the viral loads of HCV were analyzed among a cohort of voluntary blood donors who were HCV viremic. It was revealed that both the detected HCV genotypes and the donors’ gender are two independent fac.
