ted. Additional file ten. Enriched terms in up-regulated genes between Supplemented Infected vs Manage Infected. Added file 11. Enriched terms inside the list of differentially upregulated genes involving the groups Control Infected vs Supplemented Infected. Further file 12. Average liveweight and age (standard deviation) for each group in the starting and end in the experiment. Additional file 13. Composition with the handle protein and supplemented protein diets. Added file 14. Bromatological composition (g kg-1 of dry matter at one hundred ) and power (MJ) in the diets’ components. Added file 15. Bromatological analysis on the diet program. More file 16. List of ovarian RNA samples, their concentration, 260/280 and 260/230 absorbance ratios. Added file 17. EM estimation algorithm process. Further file 18. Statistical methodology used within the Enrichment analysis. Additional file 19. Protein Network evaluation. Acknowledgements Not applicable. Arrive recommendations statement This study was carried out in compliance together with the ARRIVE suggestions. Authors’ contributions P.S.H. performed the in vivo and laboratory experiments, analysed the information and wrote the manuscript. C.M.S.C. conducted the in vivo and laboratory experiments. R.C.L. performed the statistical analyses, assisted with manuscript writing and revision. D.G.C. helped with qPCR organizing and qPCR information analysis. L.M.K. cultivated and supplied the Haemonchus contortus larvae. S.M.T. assisted in the ALK1 Inhibitor custom synthesis planning of experiments. H. L. elaborated the study style and arranging, assisted within the manuscript revision. The author(s) study and approved the final manuscript. Author’s facts Paula Suarez-Henriques is usually a veterinary surgeon using a Master in Animal Science and also a Doctorate in Animal Science. She obtained each degrees inside the final six years at the University of S Paulo in Brazil. In July 2020, she presented part of her doctorate research at the Society for the Study of Reproduction virtual meeting. Before that, she worked as a veterinarian clinician as well as a investigation assistant in laboratories studying molecular pathology of breast cancers, cell and molecular biology in mammary gland improvement, and numerous myeloma resistance to alkylating agents. Funding We thank the National Council for Scientific and Technological Study -(CNPq – Brazil) and CAPES for the monetary help with Paula SuarezHenriques’ PhD studentship bursary quantity 141846/2017 and, for funding the costs of reagents and equipment necessary for the μ Opioid Receptor/MOR medchemexpress accomplishment of this research project, with method numbers CNPq 311008/2016-0 and CNPq 420373/2016. We also thank S Paulo Analysis Assistance FoundationSupplementary InformationThe online version includes supplementary material out there at doi. org/10.1186/s12917-021-03020-7. More file 1: Table S1. RNA sequencing data excellent summary. Added file two: Table S2. Number of sequences and Sequencing depth generated by RNA sequencing.Suarez-Henriques et al. BMC Veterinary Investigation(2021) 17:Web page 22 of(FAPESP) course of action numbers 2017/50451 and 2017/18086-0, for more funding for reagents. Availability of data and components The datasets employed and/or analysed through the present study are accessible in the More files. The files which are not in this section may perhaps be provided below reasonable request to Dr. Helder Louvandini e-mail address: [email protected] approval This research project, with its protocol and procedures employed, have been eth