Pplying the CL (E). EG-VEGF and VEGF expression (C) are weak or absent in the granulosa lutein cell layer. Note that VEGF is clearly expressed in this sample inside the vascular smooth muscle of some small arterioles supplying the CL (arrowheads, C). VEGFR-2 (KDR) expression is still sturdy in vessels in all layers of your CL (G). Scale bars: five mm (B); 100 m (C); 50 m (J). GL, granulosa lutein; TL, theca lutein.pattern represent a later stage of preovulatory follicle than illustrated in Figure 1, G to I, or irrespective of whether they represent an early stage of follicular atresia. CL derived from ovulatory follicles mature inside a canonical 14-day pattern.29 We examined EG-VEGF and VEGF expression inside a series of CL representing time points two days to 14 days soon after ovulation. To convey a sense of your general distribution of EG-VEGF and VEGF expression in person ovaries, autoradiographic film results of parallel sections have been digitized plus the photos corresponding to EG-VEGF and VEGF signals from representative ovarysamples were false-colored green and red, respectively. At 2 to three days just after ovulation (Figure 2; time points are inferred, in accordance with the histological criteria of Corner29), the EG-VEGF and VEGF expression resemble the pattern noticed inside the late preovulatory follicle: granulosa cells are intensely VEGF-positive, but lack important EGVEGF expression (Figure 2; C to F). At 5 days right after ovulation (Figure three), each VEGF (Figure three, C and D) and EG-VEGF (Figure 3, E and F) are strongly expressed inside a portion of granulosa lutein cells (theca lutein cells are usually not clearly distinct histologically at this stage; they may alsoVEGF and EG-VEGF in Human Ovaries 1887 AJP June 2003, Vol. 162, No.Figure five. EG-VEGF and VEGF expression in typical ovary late-regressing CL. A regressing CL (roughly day 14 after ovulation), characterized by big, pale, vacuolated theca granulosa and theca lutein cells (I, J), shows absence of both VEGF (C) and EG-VEGF (E) expression. A: False-colored autoradiographic film results show absence of VEGF (red) and EG-VEGF (green) ADAMDEC1 Proteins Molecular Weight signal in an area that microscopically corresponds to the regressing CL. Only weak VEGFR-2 (KDR) expression (G) is noted in scattered vessels inside the granulosa cell layer. A developing tertiary (antral) follicle (A and B, arrowhead) shows robust VEGF expression (see Figure 1 for facts). Scale bars: five mm (B); 100 m (C); 50 m (J).express EG-VEGF and VEGF). At 8 days following ovulation (Figure 4), EG-VEGF expression is intense within the theca lutein cells (Figure four, E and F), when VEGF expression has diminished towards the point where only weak signal remains in the peripheral thecal cells (Figure four, C and D). Figure 5 illustrates a CL undergoing involutional changes (roughly day 14 soon after ovulation). Essentially no VEGF signal is present at this stage (Figure 5; A, C, and D), and EG-VEGF expression is almost fully abolished in theca lutein cell layer (Figure 5; A, E, and F).As noted in Figures four to 7, EG-VEGF expression is regularly expressed in the ovarian stroma involving follicles, usually at decrease levels than in the theca quickly surrounding follicles, in agreement with our earlier report.18 Near the ovarian hilum, particularly strong EG-VEGF expression is detected in clusters of cells consistent with Leydig-like hilus cells30 (Figure 6). As has been previously KIR2DS1 Proteins Formulation described,31,32 these cells normally occur in intimate association with blood vessels and unmyelinated nerves (Figure 6A, closed arrowhead.
