And friction force (FF) images on the laser-patterned DLN film are shown in Figure ten. A region close to the corner with the Nourseothricin medchemexpress microcrater structure was examinedCoatings 2021, 11,12 ofto examine the friction forces around the original and laser-patterned DLN surface. Comparable towards the prior studies [25], the LFM imaging was carried out employing worn Si strategies with the tip radius of 0.5 . The friction contrast is clearly observed and characterized by considerably lower friction forces within the laser-patterned region than around the original surface, see Figure 10b. Due to somewhat deep craters, the contribution of the surface relief slope for the lateral force signal is just not fully compensated throughout subtraction of two lateral force photos [46], leading to “higher friction” at the crater edges. The reduced friction forces within the laser-patterned region are accompanied with considerably lower pull-off forces (Fpull-off ) than on the original film, as confirmed by the force istance curves (Figure 11a) measured in diverse positions inside the FF image in Figure 10b, namely: (1) Fpull-off = 1290 nN around the original film, (2) Fpull-off = 990 nN close to the region of DFHBI Epigenetic Reader Domain redeposited material, (three) Fpull-off = 63 nN in the area of redeposited material, and (4) Fpull-off = 16 nN within the center of a crater. This indicates that the ablated and redeposited material adjustments the nanoscale surface properties within and about the laser-produced microcraters. The region with the low-friction area with redeposited material covers the distance of 102 in the crater edge and, including the crater, it covers a circle area of 157 radius. The occurrence on the area “2” with slightly decrease friction and pull-off force (than on original Coatings 2021, 11, FOR PEER Critique 13 of 16 Coatings 2021, 11, xxFOR PEER Assessment 13 of to surface) is most likely triggered by mass distribution of ablated clusters/particles, major 16 variation within the structure and/or thickness with the redeposited layer.Figure 10. Surface relief (a) and friction force (b) images of your laser-patterned DLN film close to the corner of a microcrater Figure ten. Surface relief (a) and friction force (b) pictures in the laser-patterned DLN film close to the corner of a microcrater Figure 10. Surface relief (a) and friction force (b) pictures on the laser-patterned DLN film close to the corner of a microcrater structure (shown in Figure 1a), load on Si tip 120 nN. The marked points (1,2,3,four) within the image would be the locations of forcestructure (shown in Figure 1a), load onon tiptip 120 nN. The marked points (1,two,3,4) inFFimageimage will be the locations of structure (shown in Figure 1a), load Si Si 120 nN. The marked points (1,2,three,four) within the FF FF will be the areas of forcethe distancecurves measurements, shown in Figure 11. curves measurements, shown in Figure 11. distance force istance curves measurements, shown in Figure 11.Figure 11. (a) The force istance curves measured diverse points around the DLN film (marked in in the FF image in Figure Figure 11. (a) The force istance curves measured inindifferent points on the DLN film (markedthe FF image in Figure 10b): Figure 11. (a) The force istance curves measured in distinctive points on the DLN film (markedin the FF image in Figure 10b): (1) original film, (2) close to the region of redeposited material, (three) in the area of redeposited material, four) within the center 10b): (1) original film, (two) the region of redeposited material, (three) in(three) in the area of redeposited material, 4) in center of a (1) original film, (2) near near the regio.