S, was used to assess the remodeling index (lesion diameter/reference diameter), which was considered positive when the diameter at the plaque site was 10 larger than that measured in the reference segment[7,11].2)256-slice CT Scanning TechniqueCT scans were performed using a 256-slice Brilliance iCT scanner (Philips Healthcare) that features a gantry rotation time of 270 ms, resulting in a temporal resolution of 36?35 ms, depending on the heart rate of the patient and the reconstruction mode, and an isotropic sub-millimeter spatial resolution. 3) (i) Coronary calcium scoring. For the assessment of coronary calcification prospective ECG-gated non-contrast scans were performed at 75 of the cardiac cycle, and using 120 kV tube voltage and 364 mA tube current, and resultant images with a 3 mm slice thickness were used for the calculation of the Agatston score. CT Angiography (CTA) and estimation of the radiation dosage. For CTA a bolus of 80 ml of contrast 22948146 agent (Ultravist 370, Bayer Healthcare) was injected intravenously (6 ml/s). As soon as the signal in the descending aorta reached a predefined threshold of 100 HU, the scan started automatically and the entire volume of the heart was acquired during one breath-hold in(ii)Measurement of High Sensitive Troponin T (hsTnT), High Sensitive CRP (hsCRP) and HMBGBlood samples were collected from all patients within 2 hours before the CTA scans, centrifuged and serum aliquots were storedHMGB1 and Atherosclerotic Plaque Compositionat 280uC until analysis. For hs-TnT quantification an ELECSYS 2010 automated analyzer was used (Roche Diagnostics, Mannheim, Germany). The diagnostic range of this assay is 3 to 10000 pg/ml with an inter-assay coefficient of variation of 8 at 10 pg/ ml, and 2.5 at 100 pg/ml. The intra-assay coefficient of variation is 5 at 10 pg/ml and 1 at 100 pg/ml. RE-640 Hereby, based on a healthy reference population, an upper reference limit of 14 pg/ml (99th percentile for TnT) is recommended. HsCRP was quantified by nephelometry, utilizing polystyrene beadcoupled antibodies (Siemens Healthcare Diagnostics, Eschborn, Germany). HMGB1 measurement was performed using ELISA (Shino-Test Corp., Kanagawa, Japan, distributed by IBL, Hamburg, Germany) according to the manufacturer’s instructions[14] with an intra- and inter-assay coefficient of variation of ,10 .Table 1. Demographic and cardiac CT data.ParametersPatients (n = 152)DemographicsAge (yrs) Male sex 64610 87 (57 )Coronary risk factorsArterial hypertension Hypercholesterolemia Diabetes mellitus Family history of coronary artery disease Smoking Total number of risk factors (0?) 121 (80 ) 87 (57 ) 14 (9 ) 70 (46 ) 64 (42 ) 2.561.Follow-up DataPersonnel unaware of the stress results contacted each subject or an immediate family member and the date of this contact was used for calculating the follow-up time duration. Outcome data were collected from a standardized questionnaire and determined from patient interviews at the outpatient clinic or by telephone interviews. Reported clinical events were confirmed by review of the corresponding PD1-PDL1 inhibitor 1 medical records in our electronic Hospital Information Systems (HIS), contact with the general practitioner, referring cardiologist or the 
treating hospital. Death, myocardial infarction and clinically indicated coronary revascularization procedures by PCI or CABG were defined as major cardiac adverse events (MACE) during 
the follow-up period.Cardiac medicationsAspirin (100 mg/day) or clopidogrel (7.S, was used to assess the remodeling index (lesion diameter/reference diameter), which was considered positive when the diameter at the plaque site was 10 larger than that measured in the reference segment[7,11].2)256-slice CT Scanning TechniqueCT scans were performed using a 256-slice Brilliance iCT scanner (Philips Healthcare) that features a gantry rotation time of 270 ms, resulting in a temporal resolution of 36?35 ms, depending on the heart rate of the patient and the reconstruction mode, and an isotropic sub-millimeter spatial resolution. 3) (i) Coronary calcium scoring. For the assessment of coronary calcification prospective ECG-gated non-contrast scans were performed at 75 of the cardiac cycle, and using 120 kV tube voltage and 364 mA tube current, and resultant images with a 3 mm slice thickness were used for the calculation of the Agatston score. CT Angiography (CTA) and estimation of the radiation dosage. For CTA a bolus of 80 ml of contrast 22948146 agent (Ultravist 370, Bayer Healthcare) was injected intravenously (6 ml/s). As soon as the signal in the descending aorta reached a predefined threshold of 100 HU, the scan started automatically and the entire volume of the heart was acquired during one breath-hold in(ii)Measurement of High Sensitive Troponin T (hsTnT), High Sensitive CRP (hsCRP) and HMBGBlood samples were collected from all patients within 2 hours before the CTA scans, centrifuged and serum aliquots were storedHMGB1 and Atherosclerotic Plaque Compositionat 280uC until analysis. For hs-TnT quantification an ELECSYS 2010 automated analyzer was used (Roche Diagnostics, Mannheim, Germany). The diagnostic range of this assay is 3 to 10000 pg/ml with an inter-assay coefficient of variation of 8 at 10 pg/ ml, and 2.5 at 100 pg/ml. The intra-assay coefficient of variation is 5 at 10 pg/ml and 1 at 100 pg/ml. Hereby, based on a healthy reference population, an upper reference limit of 14 pg/ml (99th percentile for TnT) is recommended. HsCRP was quantified by nephelometry, utilizing polystyrene beadcoupled antibodies (Siemens Healthcare Diagnostics, Eschborn, Germany). HMGB1 measurement was performed using ELISA (Shino-Test Corp., Kanagawa, Japan, distributed by IBL, Hamburg, Germany) according to the manufacturer’s instructions[14] with an intra- and inter-assay coefficient of variation of ,10 .Table 1. Demographic and cardiac CT data.ParametersPatients (n = 152)DemographicsAge (yrs) Male sex 64610 87 (57 )Coronary risk factorsArterial hypertension Hypercholesterolemia Diabetes mellitus Family history of coronary artery disease Smoking Total number of risk factors (0?) 121 (80 ) 87 (57 ) 14 (9 ) 70 (46 ) 64 (42 ) 2.561.Follow-up DataPersonnel unaware of the stress results contacted each subject or an immediate family member and the date of this contact was used for calculating the follow-up time duration. Outcome data were collected from a standardized questionnaire and determined from patient interviews at the outpatient clinic or by telephone interviews. Reported clinical events were confirmed by review of the corresponding medical records in our electronic Hospital Information Systems (HIS), contact with the general practitioner, referring cardiologist or the treating hospital. Death, myocardial infarction and clinically indicated coronary revascularization procedures by PCI or CABG were defined as major cardiac adverse events (MACE) during the follow-up period.Cardiac medicationsAspirin (100 mg/day) or clopidogrel (7.
