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Wang C, Wang L, Geng X Higher recovery refolding of rhG-CSF from Escherichia coli, applying urea gradient size exclusion chromatography. Biotechnology Progress 24: 209213. 20. Alrokayan S Chemical synthesis and enhanced expression of recombinant human granulocyte colony-stimulating factor cDNA. Genet Mol Res 10: 2671 2678. 21. Jiang Y, Jiang W, Qiu Y, Dai W Effect of a structurally modified human granulocyte colony stimulating issue, G-CSFa, on leukopenia in mice and monkeys. J Hematol Oncol four: 28. 22. Li M, Fan H, Liu J, Wang M, Wang L, et al. Higher pH solubilization and chromatography-based renaturation and purification of recombinant human granulocyte colony-stimulating aspect from inclusion bodies. Applied Biochemistry and Biotechnology 166: 12641274. 23. Fahnert B, Lilie H, Neubauer P Inclusion bodies: formation and utilisation. Advances in Biochemical Engineering/Biotechnology 89: 93142. 24. Jeong KJ, Lee SY Secretory production of human granulocyte colonystimulating factor in Escherichia coli. Protein Expression and Purification 23: 311318. 25. Jin H, Cantin GT, Maki S, Chew LC, Resnick SM, et al. Soluble periplasmic production of human granulocyte colony-stimulating element in Pseudomonas fluorescens. Protein Expression and Purification 78: 6977. 26. Kim CW, Han KS, Ryu KS, Kim BH, Kim KH, et al. N-terminal domains of native multidomain proteins possess the possible to help de novo folding of their downstream domains in vivo by acting as solubility enhancers. Protein Science 16: 635643. 27. Song JA, Han KY, Park JS, Seo HS, Ahn KY, et al. Human G-CSF synthesis utilizing stress-responsive bacterial proteins. FEMS Microbiology Letters 296: 6066. 28. Wang Y, Wei A, Wang M, Wei X, Zhang C, et al. . Sheng Wu Gong Cheng Xue Bao 28: 13881397. 29. Lefebvre J, Boileau G, Manjunath P Recombinant expression and affinity purification of a novel epididymal human sperm-binding protein, BSPH1. Mol Hum Reprod 15: 105114. 30. Chen X, Wu J, Liu H, He Z, Gu M, et al. Approaches to efficient production of recombinant angiogenesis inhibitor rhVEGI-192 and characterization of its structure and antiangiogenic function. Protein Sci 19: 449457. 31. Busso D, Delagoutte-Busso B, Moras D Building of a set 17493865 Gatewaybased location vectors for high-throughput cloning and expression screening in Escherichia coli. Analytical Biochemistry 343: 313321. 32. Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, et al. Expression and purification of biologically active human FGF2 containing the b’a’ domains of human PDI in Escherichia coli. Applied Biochemistry and Biote.Cyte colony-stimulating aspect. EMBO Journal five: 871876. 15. Lasnik MA, Porekar VG, Stalc A Human granulocyte colony stimulating aspect expressed by methylotrophic yeast pichia pastoris. Pflugers Archiv European Journal of Physiology 442: R184186. 16. Yamamoto A, Iwata A, Saitoh T, Tuchiya K, Kanai T, et al. Expression in Escherichia coli and purification of the functional feline granulocyte colonystimulating aspect. Veterinary Immunology and Immunopathology 90: 169177. 17. Rao DV, Narasu ML, Rao AK A purification strategy for enhancing the method yield and high-quality of recombinant human granulocyte colony-stimulating aspect expressed in Escherichia coli and its characterization. Biotechnology and Applied Biochemistry 50: 7787. 18. Vanz AL, Renard G, Palma MS, Chies JM, Dalmora SL, et al. Human granulocyte colony stimulating factor: cloning, overexpression, purification and characterization. Microb Cell Truth 7: 13. 19. Wang C, Wang L, Geng X Higher recovery refolding of rhG-CSF from Escherichia coli, making use of urea gradient size exclusion chromatography. Biotechnology Progress 24: 209213. 20. Alrokayan S Chemical synthesis and enhanced expression of recombinant human granulocyte colony-stimulating element cDNA. Genet Mol Res 10: 2671 2678. 21. Jiang Y, Jiang W, Qiu Y, Dai W Impact of a structurally modified human granulocyte colony stimulating issue, G-CSFa, on leukopenia in mice and monkeys. J Hematol Oncol four: 28. 22. Li M, Fan H, Liu J, Wang M, Wang L, et al. Higher pH solubilization and chromatography-based renaturation and purification of recombinant human granulocyte colony-stimulating aspect from inclusion bodies. Applied Biochemistry and Biotechnology 166: 12641274. 23. Fahnert B, Lilie H, Neubauer P Inclusion bodies: formation and utilisation. Advances in Biochemical Engineering/Biotechnology 89: 93142. 24. Jeong KJ, Lee SY Secretory production of human granulocyte colonystimulating factor in Escherichia coli. Protein Expression and Purification 23: 311318. 25. Jin H, Cantin GT, Maki S, Chew LC, Resnick SM, et al. Soluble periplasmic production of human granulocyte colony-stimulating element in Pseudomonas fluorescens. Protein Expression and Purification 78: 6977. 26. Kim CW, Han KS, Ryu KS, Kim BH, Kim KH, et al. N-terminal domains of native multidomain proteins have the potential to help de novo folding of their downstream domains in vivo by acting as solubility enhancers. Protein Science 16: 635643. 27. Song JA, Han KY, Park JS, Seo HS, Ahn KY, et al. Human G-CSF synthesis employing stress-responsive bacterial proteins. FEMS Microbiology Letters 296: 6066. 28. Wang Y, Wei A, Wang M, Wei X, Zhang C, et al. . Sheng Wu Gong Cheng Xue Bao 28: 13881397. 29. Lefebvre J, Boileau G, Manjunath P Recombinant expression and affinity purification of a novel epididymal human sperm-binding protein, BSPH1. Mol Hum Reprod 15: 105114. 30. Chen X, Wu J, Liu H, He Z, Gu M, et al. Approaches to efficient production of recombinant angiogenesis inhibitor rhVEGI-192 and characterization of its structure and antiangiogenic function. Protein Sci 19: 449457. 31. Busso D, Delagoutte-Busso B, Moras D Construction of a set 17493865 Gatewaybased location vectors for high-throughput cloning and expression screening in Escherichia coli. Analytical Biochemistry 343: 313321. 32. Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, et al. Expression and purification of biologically active human FGF2 containing the b’a’ domains of human PDI in Escherichia coli. Applied Biochemistry and Biote.
